Abstract
We present a microsystem lab chip with high throughput continuous cell separation and focus via dielectrophoresis (DEP) based on particle characterization. The experiments are demonstrated with polystyrene (PS) beads 8 μm, 25μm and HMEC-1 cells (and HepG2 cells) in suspension DEP buffer solution. Under a voltage 10 V peak-peak and frequency 100 kHz, different characteristics particles have different DEP forces. Thus, we have different deflections that mean sample is separated. Then, cells and particles are focused for collection or optical detection application.