Abstract
We for the first time report the development of a DNA detection method which can quantify zept-mole level DNA by combination of Thermal Lens Microscope (TLM) and Rolling Circle Amplification (RCA). In this method, the target DNA is extended by RCA, and then the RCA products are detected by TLM, making use of high selectivity of RCA and high sensitivity of TLM. This method is more highly specific than PCR and the detection limit is 22 zeptmol. This study is contributable to cancer diagnosis and detection of viruses.