INTEGRATED MICROFLUIDIC LOOP-MEDIATED-ISOTHERMAL-AMPLIFICATION SYSTEMS FOR  RAPID ISOLATION AND DETECTION OF AQUACULTURE PATHOGENS,

Wen-Hsin Chang; Sung-Yi Yang; Chih-Hung Wang; Ming-An Tsai; Pei-Chyi Wang; Shih-Chu Chen; Tzong-Yueh Chen; Gwo-Bin Lee

doi:10.1109/TRANSDUCERS.2011.5969305

Conference paper

INTEGRATED MICROFLUIDIC LOOP-MEDIATED-ISOTHERMAL-AMPLIFICATION SYSTEMS FOR RAPID ISOLATION AND DETECTION OF AQUACULTURE PATHOGENS,

Wen-Hsin Chang, Sung-Yi Yang, Chih-Hung Wang, Ming-An Tsai, Pei-Chyi Wang, Shih-Chu Chen, Tzong-Yueh Chen and Gwo-Bin Lee

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, pp.210-213

2011

DOI: https://doi.org/10.1109/TRANSDUCERS.2011.5969305

Abstract

aquaculture pathogens;Loop-mediated-isothermal-amplification;microfluidics

Detection of fish pathogens is crucial for premium aquaculture species with a high economic value. The current study therefore presents a new microfluidic system for detection of aquaculture pathogens. Pathogen DNA was first purified by using specific nucleotide probes, followed by amplifying pathogen-specific DNA fragments by adopting the loop-mediated-isothermal-amplification (LAMP) process. Furthermore, it can carry out four LAMP reactions for multiple detection of pathogens. Successful demonstration for detection of aquaculture fish pathogens has been achieved by using the proposed microfluidic system. © 2011 IEEE.

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Title: INTEGRATED MICROFLUIDIC LOOP-MEDIATED-ISOTHERMAL-AMPLIFICATION SYSTEMS FOR RAPID ISOLATION AND DETECTION OF AQUACULTURE PATHOGENS,
Translated title: INTEGRATED MICROFLUIDIC LOOP-MEDIATED-ISOTHERMAL-AMPLIFICATION SYSTEMS FOR RAPID ISOLATION AND DETECTION OF AQUACULTURE PATHOGENS,
Creators - without role: Wen-Hsin Chang - National Tsing Hua University
Sung-Yi Yang - National Tsing Hua University
Chih-Hung Wang - National Tsing Hua University
Ming-An Tsai - National Pingtung University of Science and Technology
Pei-Chyi Wang - National Pingtung University of Science and Technology
Shih-Chu Chen - National Pingtung University of Science and Technology
Tzong-Yueh Chen - National Cheng Kung University
Gwo-Bin Lee - Department of Power Mechanical Engineering , National Tsing Hua University
Publication Details: 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, pp.210-213
Identifiers: 9781457701573; 9957770042306774
Academic Unit: Institute of Biomedical Engineering, College of Engineering, National Tsing Hua University
Language: English
Resource Type: Conference paper

INTEGRATED MICROFLUIDIC LOOP-MEDIATED-ISOTHERMAL-AMPLIFICATION SYSTEMS FOR RAPID ISOLATION AND DETECTION OF AQUACULTURE PATHOGENS,

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