Abstract
The study presents a new microfluidic flow cytometer system integrated with functional micro-devices for bead-based immunoassay capable of viral samples pretreatment and detection. Several essential components are integrated into a single chip including a microfluidic flow cytometer device, a bio-samples pretreatment module with the microcoils for concentration by using antibody-conjugated magnetic beads and an optical control device to perform the functions of virus sample injection, optical signal detection, and virus collection. The virus sample with a concentration of 10 4 pfu/ml can be detected successfully by the optical control device in the proposed system. Consequently, the miniature microfluidic flow cytometer system may provide a powerful platform for future biological applications.