Abstract
We present a simple method that can deliver homogeneous controllable stress at regular vibration cycle by incubating rabbit articular chondrocyte primary cells on polydimethylsiloxane (PDMS) micro wells. To promote the adhesion of cells on PDMS surface, both oxygen plasma and collagen treatments are employed on the PDMS chip surface. Uniform cells were grown inside the wells and underwent different stress and cycling conditions, and it was found that a 3% strain at 0.5Hz continuously stretching of 1 hours for two days was most helpful for bone cells growth.