Abstract
This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods. © 2012 IEEE.