Integration of immunoassay into extended nanospace for analysis at single-molecule level

Fukashi Hiruma; Kazuma Mawatari; Takehiko Tsukahara; Takehiko Kitamori

Conference paper

Integration of immunoassay into extended nanospace for analysis at single-molecule level

Fukashi Hiruma, Kazuma Mawatari, Takehiko Tsukahara and Takehiko Kitamori

12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, pp.221-223

2008

Appears in keyword about Chemistry

Abstract

ELISA

Extended-nano

Single cell

TLM

Chemical Engineering (miscellaneous)

Bioengineering

We report a novel ELISA (Enzyme-Linked ImmunoSorbent Assay) system utilizing extended nanospace (3μm wide and 300 nm deep) for analysis at singlemolecule level. Nanofabrication, nanofluidic system and detector (thermal lens microscope, TLM) were realized. Repeatable immunoassay format was developed by chemisorption of capture antibody at glass wall. The performance was evaluated, and LOD of 20 molecules (60 pL sample volume) were verified. This system will become powerful tool for single cell analysis due to the quite small volume. © 2008 CBMS.

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Title: Integration of immunoassay into extended nanospace for analysis at single-molecule level
Translated title: Integration of immunoassay into extended nanospace for analysis at single-molecule level
Creators - without role: Fukashi Hiruma - Department of Applied Chemistry, School of Engineering, University of Tokyo
Kazuma Mawatari - Kanagawa Academy of Science and Technology
Takehiko Tsukahara - RLNR, Tokyo Institute of Technology
Takehiko Kitamori - Department of Applied Chemistry, School of Engineering, University of Tokyo
Publication Details: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, pp.221-223
Identifiers: 9957768453606774
Academic Unit: Institute of NanoEngineering and Microsystems, College of Engineering, National Tsing Hua University
Language: English
Resource Type: Conference paper

Integration of immunoassay into extended nanospace for analysis at single-molecule level

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