Abstract
We integrated the pre-preparation processes of single cell at picoliter scale on a quartz silica microchip. The processes contain single cell trap and separation into picoliter volume liquid, injection of cell lysis buffer of sub-picoliter volume, and injection of single cell lysate into the femtoliter volume space. Single cell was separated into 7 picoliter liquid by the use of chamber structure and chemically lysed with minimum dilution. Lysed sample was injected into the extended-nano channel by pressure. The developed method enabled manipulating samples extracted from single cell from picoliter to femtoliter scale and is useful for single-cell protein analysis. Copyright © (2011) by the Chemical and Biological Microsystems Society.