Abstract
We have succeeded in investigating enzyme reaction in extended-nano (10-1000 mil) space modified by phospholipid bilayers which mimics an intercellular structure (from several tens to several hundreds nanometer-spacing). Previously, enzyme reaction in intercellular space was difficult to detect both in vivo and in vitro. However, this analytical model enables to investigate enzyme reaction in intercellular space, and will be powerful tools for study 011 cell functions close to cell membranes.