Isoelectric focusing in a micro chamber array

Ryo Ishibashi; Takehiko Kitamori; Kiyohito Shimura

Conference paper

Isoelectric focusing in a micro chamber array

Ryo Ishibashi, Takehiko Kitamori and Kiyohito Shimura

Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007, pp.1423-1425

2007

Appears in keyword about Chemistry

Abstract

Electrophoresis

Isoelectric focusing

Protein purification

Protein separation

Chemical Engineering (miscellaneous)

Bioengineering

We have developed a chip-based protein fractionation device, in which two-step isoelectric focusing results in the development of a pH gradient in a square array of hundred chambers (140 nL each). Test samples were fractionated into each chamber, in a total focusing time of less than 30 min.

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Title: Isoelectric focusing in a micro chamber array
Translated title: Isoelectric focusing in a micro chamber array
Creators - without role: Ryo Ishibashi - School of Engineering , University of Tokyo
Takehiko Kitamori - School of Engineering , University of Tokyo
Kiyohito Shimura - School of Engineering , University of Tokyo
Publication Details: Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007, pp.1423-1425
Identifiers: 9780979806407; 9957776223306774
Academic Unit: Institute of NanoEngineering and Microsystems, College of Engineering, National Tsing Hua University
Language: English
Resource Type: Conference paper

Isoelectric focusing in a micro chamber array

Abstract

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