Abstract
Recently, the requirement for living single cell analysis is rapidly increasing because of cell heterogeneity in cell biology and medical fields. However, single cell volume is ∼pL and too small to analyze by conventional method. Based on this consideration, we realized culture and release of single cell in microchip after fL sampling by micro/extended-nano interface. The results show that the cell maintained viability during long period culture after sampling. This study provides important information for living single cell dynamic process during cell culture after fL sampling.