Microchip integrated rolling circle amplification for single DNA molecule detection in minute sample volumes

Atsuki Tachihara; Kae Sato; Björn Renberg; Yuki Tanaka; Jonas Jarvius; Mats Nilsson; Takehiko Kitamori

Conference paper

Microchip integrated rolling circle amplification for single DNA molecule detection in minute sample volumes

Atsuki Tachihara, Kae Sato, Björn Renberg, Yuki Tanaka, Jonas Jarvius, Mats Nilsson and Takehiko Kitamori

12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, pp.426-428

2008

Appears in keyword about Chemistry

Abstract

DNA

Rolling circle amplification

Solid-phase amplification

Chemical Engineering (miscellaneous)

Bioengineering

We have recently successfully integrated Rolling Circle Amplification (RCA) into microchannels in microchips using bead immobilized DNA oligos. Using RCA amplification, sensitivity was increased to detection of only 9000 molecules, which is not possible using flow cytometry based methods, and is an important step towards single analyte molecule detection. © 2008 CBMS.

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Title: Microchip integrated rolling circle amplification for single DNA molecule detection in minute sample volumes
Translated title: Microchip integrated rolling circle amplification for single DNA molecule detection in minute sample volumes
Creators - without role: Atsuki Tachihara - The University of Tokyo
Kae Sato - The University of Tokyo
Björn Renberg - The University of Tokyo
Yuki Tanaka - Uppsala University
Jonas Jarvius - Uppsala University
Mats Nilsson - Uppsala University
Takehiko Kitamori - The University of Tokyo
Identifiers: 9957771530606774
Academic Unit: Institute of NanoEngineering and Microsystems, College of Engineering, National Tsing Hua University
Language: English
Resource Type: Conference paper
Publication Details: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, pp.426-428

Microchip integrated rolling circle amplification for single DNA molecule detection in minute sample volumes

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