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Microfluidic immunochemical test strip with oligonucleotide-labeled antibody
Conference paper

Microfluidic immunochemical test strip with oligonucleotide-labeled antibody

Y. Oku, S. Akaba, R. Hara, M. Umeda, M. Tokeshi and T. Kitamori
Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Vol.1, pp.1321-1323
2005

Abstract

Diagnosis Immobilization Immunoassay Thermal lens microscopy Chemical Engineering (miscellaneous) Bioengineering
This paper reports a new on-chip immunological detection system that was detected Hepatitis B surface antigen (HBsAg). The channel fabricated on a chip has unique structures. In order to increase binding efficiency, oligonucleotide (ON) is immobilized on the wall of a shallow part of the channal. And, to enhance detection sensitivity, the depth of a deep part is optimized for the thermal lens measurement. Using this system, we succeeded in detecting 10 pg/mL of HBsAg. Copyright © 2005 by the Transducer Research Foundation, Inc.

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