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Mitochondrial and genomic DNA damage analysis in microfluidic chips
Conference paper

Mitochondrial and genomic DNA damage analysis in microfluidic chips

Yuki Tanaka, Henrik Johansson, Chatarina Larsson, Jonas Jarvius, Takehiko Kitamori and Mats Nilsson
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, pp.874-876
2008
Appears in  keyword about Chemistry

Abstract

DNA damage analysis In situ Single cell Single molecule Chemical Engineering (miscellaneous) Bioengineering
This paper reports analysis of DNA damage, both in mitochondrial DNA (mtDNA) and nuclear DNA in single cells using a microfluidic platform and padlock probes. Damage in DNA such as strand breaks were analyzed in situ using padlock probes, made visible by rolling-circle amplification (RCA) of the circularized padlock probes [1]. The correlation between DNA damage / damage repair and single nucleotide variation in individual mitochondrial DNA also have been studied. © 2008 CBMS.

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