Abstract
We report a novel measurement method of velocity distribution for 100 nm channels employing sizeregulated fluorescent single molecules as flow tracer, i.e., molecular image velocimetry (MIV). MIV enables study of fluid flow in nanochannels, which has been difficult for conventional particle image velocimetry (PIV) employing 100 nm particles of similar size to the channel. This method will further understanding of nanoscale fluid dynamics important for nanofluidics.