Abstract
In this report, we developed an extended-nano (10-1000 nm) fluidic network to realize continuous flow chemical processing (CFCP) for single cell secretion analysis. Firstly, equivalent mixing was demonstrated in the network by optimizing fabrication conditions and applied pressure. Secondly, chemical reaction was realized in the network, and the products were detected by differential interference contrast thermal lens microscope (DIC-TLM). This is the smallest reaction channel network, and will be applied for single cell activity analysis. Copyright © (2011) by the Chemical and Biological Microsystems Society.