Abstract
Single cell analysis is potentially beneficial to understand cell to cell behavior with their intracellular compounds from population of millions of cells together. We demonstrate an efficient and fast method for multi-localized single cell electroporation, where electroporation take place with multiple positions together at a tens nanometer confined region on single cell membrane by using ITO nano-electrode arrays. In this study, we successfully deliver dyes and protein into single cell with different field strengths and pulse durations. This new approach can allow to analyze different dyes/molecules interaction in single cell which potentially applicable for medical diagnostics and biological studies.