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Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury
Conference paper

Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury

Ting-Hsu Chen, Chien-Chong Hong, Tong-Miin Liu, Chian-Lang Hong, Chung-Hang Wang, Chih-Chung Lin and Yun-Ching Huang
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Vol.2, pp.832-834
2018

Abstract

Acute kidney injury Electrical biosensor Microfluidic chip Molecularly-imprinted polymer Neutrophil gelatinase-associated lipocalin protein Chemistry (all) Bioengineering Chemical Engineering (miscellaneous) Control and Systems Engineering
In this paper, we present novel electrical microfluidic biosensors with artificially-made recognition elements for detection of Neutrophil gelatinase-associated lipocalin (NGAL) protein, which is a biomarker of acute renal failure (AKI). By using molecularly-imprinted polymer electricpolimerized on electrode, we can identify different concentration of NGAL protein. The results shows that in the specific region of current density and voltage, the polymerized electrodes can distinguish different concentrations of protein well. It can also recognize concentrations in ppb level(50~600 ng/mL). Also, the performance of sweeping frequency reveals low frequency can show more capacitance difference in all concentration, may promote the detect efficiency.

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