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Novel detection of non-absorbing molecules by optical near-field induced Thermal Lens Microscopy
Conference paper

Novel detection of non-absorbing molecules by optical near-field induced Thermal Lens Microscopy

T.H.H. Le, K. Mawatari, H. Shimizu, T. Yatsui, T. Kawazoe, M. Naruse, M. Ohtsu and T. Kitamori
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Vol.1, pp.675-677
2013

Abstract

Detection of non- absorbing molecules Differential interference contrast TLM (DIC-TLM) Nanofabrication Optical Near-Field (ONF) Bioengineering
This study reports a novel detection method of Thermal Lens Microscopy (TLM), in which the excitation of analytes is manipulated by Optical Near-Field (ONF) generated on nanostructures. Owning to the property of apparent higher energy than incident photons, herein the ONF allows the electronic excitation of molecules even though the energy of incident light is lower than absorption energy of molecules. As a result, the detection of UV-absorbing molecules has been realized with visible excitation. Our result promises important implications in detection of biomolecules, which usually have absorption in UV range. It is also worthy to note that the present work is novel in using ONF on nanostructures of non-metal materials, which is conceptually different from the plasmonic field enhancement on metal nanoparticles. This study is expected to open a new perspective for ONF-induced spectroscopies realized by top-down nanofabrication. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved. All rights reserved.

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