Abstract
To quantify biomolecules in samples with an enzyme-linked immunosorbent assay (ELISA) with smaller sample volumes more rapidly and easily, we developed a fully automated system called "μELISA™" that uses microfluidics and highly sensitive thermal lens detection. We applied μELISA™ to a one-step sandwich immunoassay of thyroid-stimulating hormone (TSH) for diagnosing thyroid function. We evaluated its performance using human serum samples and demonstrated that it is fast (15 min), highly sensitive (0.1-10 μIU-mL ), highly precise (<8% variation), and easy to operate. As a result, our system is a potentially powerful and practical diagnostic tool for point-of-care testing (POCT).