Abstract
Compact and ultra-sensitive immunoassay with fast response is a most attractive topic in microfluidic applications of bioassay, medical diagnosis, etc. In this research we present thin-layered ELISA microchip with immobilized antibody which can be stored both in dry and wet conditions. We optimized the immobilization conditions and confirmed 1 month stability of the assay in dry conditions and 7 month stability in wet conditions and demonstrated high sensitivity of the developed method.