Abstract
We introduce a facile system to deliver molecules in 3D spheroids, generated via hanging drop cell culture mixed with nano-spikes gold-nanoparticles (ns-AuNPs) and activated by nanosecond pulsed laser mediated optoporation. Due to pulse laser interaction on hanging drop cell culture with ns-AuNPs, the cell membrane deforms, creating transient membrane pore for biomolecular delivery into cells. We aim to establish an advanced high throughput drug delivery model and screening through hanging drop spheroid cell culture derived from cervix cancer cell line SiHa with Propidium Iodide (PI) dye delivery. The maximum delivery efficiency and cell viabilities were achieved of 90% and 97%.