Abstract
Single cell analysis has difficulties due to small sample volume as picoliter. Extended nanospace(10-1000 nm) which has been studied by our group has femtoliter to attoliter volume smaller than single cells and is applicable for the analysis. However, solid phase capture of analytes at defined positions for detection has not yet realized. Here, we developed a novel patterning method of biomolecules in extended nanospace by using low temperature bonding of fused silica, which avoids destruction of organic molecules by thermal bonding. Patterned probe DNA showed good selectivity and stability, and fast hybridization time was observed in nanochannels. The method can be applied to immunoassays for protein quantification.