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Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples
Conference paper

Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples

Ken Oikawa, Akihide Hibara and Takehiko Kitamori
Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp.1277-1279
2006

Abstract

Absorbance Photothermal Visualization Chemical Engineering (miscellaneous) Bioengineering
This paper describes the development of a non-scanning photothermal imaging method by CCD-based optics. By utilizing visible excitation laser and lamp-based illumination with phase-contrast optics, image-acquiring time was 4 min for 100 μm × 100 μm areas. In the present stage, the system could detect 7.5 × 10 absorbance while normal microscope can visualize 10 absorbance. © 2006 Society for Chemistry and Micro-Nano Systems.

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