Abstract
Plasma separation from minute volume of whole blood is essential technnologies. Microchip has a potential to realize it. However, highly separation efficiency and cell-free plasma separation from whole blood has not been realized yet. We focused on radial migration of blood cells by Saffman force, which depends on shear rate. A microchip was designed by considering shear rate and pressure balance between blood channel and plasma channel for preventing hemolysis. Separation efficiency of 65 % was realized without hemolysis and blood cells. Furthermore, the separation plasma was connected with an automatic micro-ELISA system and analyzed. © 2009 CBMS.