Abstract
An automated full-range quantitation method for identifying d-methamphetamine (d-MA) in human hair using a microchip-based ELISA system (microELISA) in combination with a micropulverized extraction method for sample preparation has been developed. An anti-d-MA antibody and a peroxidase-linked MA were used for the competitive ELISA assay. Method validation was carried out using doped hair samples, and segmental analyses of real-case specimens were carried out by both microELISA and liquid chromatography/tandem mass spectrometry (LC-MS/MS) to verify the reliability and applicability of this new method. Sample preparation and quantitation can be accomplished easily and quickly (less than 30 min) in smallscale contamination-free environments. © 2008 CBMS.