Abstract
Detection of localization of kinases within cells has been largely carried out based on imaging of a low number of cells or subcellular fractionation/Western blotting which provide only the average behaviors of cell populations. Here we demonstrate a new tool, referred to as microfluidic electroporative flow cytometry (EFC), to detect the translocation of an EGFP-tagged tyrosine kinase, Syk, to the plasma membrane in B cells at single cell level. Due to the frequent involvement of kinase translocations in disease processes such as oncogenesis, our approach will have utility for kinase-related drug discovery and tumor diagnosis and staging.