Abstract
In this research, we design a micro-drug-carrier by using micro-Electrospray ionization and surface cross linking process to elongate the delivery time of micro-drug carriers. The technology can be used in vaccine delivery on preparing a sustained-release system to extend the stimulation time for boosting immune response. By crosslinking the alginate-calcium particles with chitosans of different molecular weights, the chitosan crosslinked particle has a more stable particle structure, which not only reduces the leakage by 3 folds (75% to 25%) and extends a sustainable time for more than 30 days from 1 day, suitable for the application of sustainable vaccine delivery.