Single DNA molecule detection by on-bead rolling circle amplification in a microchip

Atsuki Tachihara; Kae Sato; Kiichi Sato; Yuki Tanaka; Jonas Jarvius; Mats Nilsson; Takehiko Kitamori

Conference paper

Single DNA molecule detection by on-bead rolling circle amplification in a microchip

Atsuki Tachihara, Kae Sato, Kiichi Sato, Yuki Tanaka, Jonas Jarvius, Mats Nilsson and Takehiko Kitamori

Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007, pp.53-55

2007

Appears in keyword about Chemistry

Abstract

DNA

Rolling circle amplification

Solid-phase amplification

Chemical Engineering (miscellaneous)

Bioengineering

This paper reports a single DNA molecule detection by on-bead rolling circle amplification (RCA) in a microchip. RCA was successfully done in the microchip, and individual RCA products on beads were visible with a fluorescence microscope as a bright object, and they could be easily counted.

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Title: Single DNA molecule detection by on-bead rolling circle amplification in a microchip
Translated title: Single DNA molecule detection by on-bead rolling circle amplification in a microchip
Creators - without role: Atsuki Tachihara - The University of Tokyo
Kae Sato - The University of Tokyo
Kiichi Sato - The University of Tokyo
Yuki Tanaka - Uppsala University
Jonas Jarvius - Uppsala University
Mats Nilsson - Uppsala University
Takehiko Kitamori - The University of Tokyo
Publication Details: Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007, pp.53-55
Identifiers: 9780979806407; 9957777368106774
Academic Unit: Institute of NanoEngineering and Microsystems, College of Engineering, National Tsing Hua University
Language: English
Resource Type: Conference paper

Single DNA molecule detection by on-bead rolling circle amplification in a microchip

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