Abstract
We integrated ELISA (Enzyme-Linked Immunosorbent Assay) in nanochannels for quantitative analysis at the single-molecule level. In the single molecule analysis, losing even single target molecule is critical. However, causes of losses was unclear. Here, we investigated and minimized losses by developing nanofluidic ELISA devices. We realized quantification of C-reactive protein (CRP) with the limit of detection (3.3V) of 17 molecules.