Abstract
Molecular transport in 100 nm inter/intra cellular spaces such as synaptic clefts and mitochondria has important roles in biological functions. Our group has developed a powerful in vitro tool, i.e., bio-mimetic extended nanospace (10-1000 nm), lipid bilayer-modified extended nanochannel for mimicking the cellular spaces. In this study, we have revealed liquid viscosity and molecular diffusion in bio-mimetic extended nanospace. The diffusion of proteins and ions was much slower by increased viscosity of confined liquid, which is considered to be induced by loosely coupled water molecules within 50 nm of the surface.