Abstract
Seasonal and novel influenza infections have caused worldwide pandemic. In order to properly treat the infected patients, a rapid and accurate influenza diagnostic tool should be developed. This study therefore presented a new integrated microfluidic system for detection of influenza infection. It integrated a suction-type, pneumatic-driven microfluidic control module, a magnetic bead-based fluorescent immunoassay (FIA) and an end-point optical detection module. This new system could successfully distinguish the influenza A and B within 15 minutes, respectively. Furthermore, the results from 40 patient specimens have concluded that 75% sensitivity and 75% specificity in both positive and negative detections. This developed system would provide a powerful platform for fast screening of influenza infection. Copyright © (2011) by the Chemical and Biological Microsystems Society.