Abstract
We report here a new on-chip immunological assay system that can detect 100 pg/mL of Hepatitis B surface antigen (HBsAg) within 15 minutes by using a premixed reagent mixture and a semi-automated analyzer. This system is used only two reagents. One reagent is antigen-antibody reaction-related contents and the other reagent is an enzyme substrate. Instead of an antibody-immobilization in the microchannel, biotinyl oligonucleotide-immobilized microchannel was used for antigen trapping. As a result, our assay system required less time than the conventional step-by-step method. © 2006 Society for Chemistry and Micro-Nano Systems.