Abstract
Polymeric nanoparticle-based temperature measurement and imaging is an emerging field of biomedical applications. Herein, the hydrophilic Rhodamine dye molecules were bound with the Pluronic F127 molecule and subsequently incorporated in the cross-linked urea paraformaldehyde (UPF) resin that forms nanoparticles names as Urea-ParaFormaldehyde Rhodamine incorporated nanoparticles (UPFRNP) during high-temperature cross-link treatment. UPFRNP dispersed in water presenting a size range of 160±30 nm, the zeta potential was -18 mv. UPFRNP is highly stable in severe treatments such as autoclave sterilization and lyophilization. Also, UPFRNP is biocompatible and fit for biological applications such as PCR, intracellular temperature measurements.