Abstract
Objective: This study investigated the effects of antrodia camphorata (AC) supplementation on anti-fatigue effects after a single bout of exhaustive exercise in rats. Methods: Thirty-two rats were randomly divided into four groups: control (C, n=8); C with antrodia camphorata supplementation (A, n=8); exhaustive exercise (E, n=8); exhaustive exercise with AC supplementation (AE, n=8). Rats in groups A and AE received a 0.2 c.c. (kg/wt/day) AC supplementation for two weeks. Rats in groups E and AE performed an exhaustive running test on a treadmill at a final speed of 30m/min, 10% grade, at approximately 70-75% VO2max. Blood samples of each rat were collected. The concentrations of glucose, lactate dehydrogenase (LDH), uric acid, creatinine, and creatine kinase (CK) in blood were examined. Results: The results showed a significant increase in exhaustive exercise time of AE group (78.79±9.09 min) compared with E group (67.77±9.22 min). The activities of CK, LDH, and blood creatinine concentration significantly increased in exercised rats (1227.40±553.24 U/I, 2108.32±513.99 U/I, 0.76±0.19 mg/dl) compared with control rats (358.98±125.53 U/I, 956.89±597.13 U/I, 0.5±0.15 mg/dl). The increased activities of CK, LDH, and creatinine concentration in exercised rats (1227.40±553.24 U/I, 2108.32±513.99 U/I, 0.76±0.19 mg/dl) were significantly decreased in exercised rats supplemented with AC (774.13±203.38 U/I, 1300.93±513.99 U/I, 0.5±0.05 mg/dl). The glucose in the plasma significantly decreased in exercised rats (133.88±61.84mg/dl) compared with C rats (209.63±69.13 mg/dl). The glucose concentration was significantly increased in AE group (188.88±60.32 mg/dl) compared with E group (133.88±61.84 mg/dl). Conclusion: The results indicate that AC supplementation could decrease CK, LDH activities. It’s help for anti-fatigue effects.