Abstract
In this paper, we have demonstrated the trapping single cell into the microfluidic dead zone by using optoelectronic tweezers (OET) in the poly (ethylene glycol)-based U-shaped microwells for the study of the immune activity. Using light-induced dielectrophoresis (DEP) on a photoconductor, virtual electrode pattern was generated by projecting an LED light through a DMD spatial light modulator. We have shown a target cell had trapped by the donut shape of the OET force and PEG-based U-shaped microwells. Based on our experiment, we provided an easily performed method for study the interaction of NK cells and target cells. By the way, we had evaluated the NK cell activity in single cell level by the PEG-based OET chip.