摘要
Dengue fever is one of the acute flavivrus-borne infectious diseases caused by dengue virus with four serotypes. To date, there are no efficient diagnostic tools available for monitoring the disease activity of dengue fever. It is needed to develop a diagnostic device with the characteristics of inexpensiveness, ease-to-use and robustness for the detection of dengue fever. In this study, we have developed an ELISA-based diagnostic device prepared via wax printing method through using filter paper. The antigens that we targeted to quantify in the buffer system were both non-structural protein 1 with the detection limit of about 100 pg/mL and envelope protein with the diluted virus culture soup of about 100 times of serotype 2 dengue virus (only 2 μL sample). We believe that this study would provide insight on the development of in-vitro diagnostic devices for dengue fever and various diseases in the different divisions of medicine. © 2013 IEEE.