摘要
Folate receptor alpha (FRα) has been regarded as a promising target for cancer therapy. Several antibody-based anti-cancer drugs have been developed; however, no inhibitory aptamer, which is more stable and economical, has been screened. In this study, we screened FRα-specific inhibitory aptamers by using a new integrated microfluidic system which was capable of precise control of the shear force (up to nN) that allowed high-affinity ssDNA to be collected and screened. With this optimized approach, the screened aptamer could serve as a great candidate for therapy. © 2023 IEEJ.