Abstract
ABSTRACT Activation of hydrogen peroxide using transition metal ions/complexes is so-called Fenton/Fenton-like reaction since 1876. The utilization of hydrogen peroxide catalyzed by transition metal-complexes or natural redox metalloenzyme has been widely applied in different disciplines, such as: chemical mechanical polishing (CMP) process in integrated circuit (IC) industry, polymerization synthesis in traditional industries, and the treatment of wastewater in the environmental concerns (which is called “advanced oxidation process”). The activation process was proposed to transform hydrogen peroxide into hydroxyl radicals or other active species called reactive oxygen species (ROS). Besides engineering science, Fenton/Fenton-like reactions play a very important role in the mechanism of aging and disease induction for its capacity to destroy DNA, peptide in tissue/organs. Based on the objectives of investigation on hydrogen peroxide activation, reaction pathways and its utilization, we have divided our study into two parts: the first part is to focus on investigating the catalytic efficiency of different copper(II)- amino acid complexes on peroxide activation and its possible reacting pathways; then, we make efforts to discuss the inferences of system additives, including salts, buffer, radicals, and antioxidants. We chose amino acids, the essential unit to compose natural matters, as the target chelating compounds. The main goal is to see if hydroxyl radical really dominate the reaction mechanism as that of traditional Fenton reaction. The second part is to immobilize one of redox metalloenzymes, horse radish peroxidase, into silica gel using one-step sol-gel technique and then to test its function towards the development of optical catalytic enzymatic reactor or flow-through reactor. Different silica precursors and silica surface modifiers are tested for obtain optimized composition to fabricate mesoporous/ macroporous materials. Meanwhile the biocom- patibility was compared among different fabricated silica gels using HRP activity performance as the indicator. After all, more efforts are expected to develop an economical, on line-LC or on-line CE ROS and antioixdant sensing system, which complete quantification and quantitation analysis concurrently.