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用基因表現圖譜分辨傳統中醫的四逆湯證
Dissertation

用基因表現圖譜分辨傳統中醫的四逆湯證

鄭宏足
Doctor of Philosophy (PHD), 國立清華大學, 奈米工程與微系統研究所
2015

Abstract

四逆湯證 傳統中醫 基因表現圖譜 基因微陣列晶片 生物標記基因 Sini decoction pattern (SDP) traditional Chinese medicine (TCM) ZHENG cDNA microarray gene expression profiling biomarkers
We investigated the syndromes of the Sini decoction pattern (SDP). To obtain a common profile for SDP, we distributed questionnaires to 300 clinical traditional Chinese medicine practitioners. According to the survey, we concluded 2 sets of symptoms for SDP: (1) pulse feels deep or faint and (2) reversal cold of the extremities. Then we collected 24 individuals, and extracted their total mRNA of peripheral blood mononuclear cells for cDNA microarray experiments. 12 samples were used as the training set to identify the biomarkers for distinguishing the SDP and non-SDP groups. The remaining 12 samples were used as the test set. The test results indicated that the gene expression profiles of the identified biomarkers could effectively distinguish the 2 groups.

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