Abstract
Phosphorus (P) is one of the seventeen essential elements for plant growth, including roots formation and seed germination. It is high importance for cellular components, such as phospholipids for membrane and phosphoproteins for regulation of enzymatic processes in the cell. Plant vacuoles are the largest organelles occupying 90% volume of a plant cell. It have multiple functions including storage of small molecules such as minerals, water, toxic compounds and metabolites. The charge transporters localized in the vacuolar membranes have two types: cation transporters, such as vacuolar Ca2+ transporters and vacuolar Na+/K+ transporters, and anion transporters, such as SPX-MFS families. The molecular mass of Oryza sativa SPX-MFS1, is about 78.7 kDa. From the MEMSAT-SVM analysis, Oryza sativa SPX-MFS1 has twelve transmembrane helices. We would focus on overexpression of Oryza sativa SPX-MFS1 in the heterologous expression system using Saccharomyces cerevisiae (BJ2168) as a host and on the isolation of the yeast microsome containing this protein. We have obtained OsSPX-MFS1 and tried to analyze it for future in vitro research, like transport mechanism and 3-dimension structure determination.