Abstract
Abstract The establishment of cellular compartment boundary is a general phenomenon in animal development. Separating cells into discrete compartment is important to prevent mislocated cell signaling and is crucial for cell patterning, morphogenesis, and organogenesis. In Drosophila wing imaginal disc, the anterior-posterior compartment boundary is characterized with straight and smooth border, which can be visualized when fluorescent-marked clone cells of one compartment touch the boundary. To date, it’s believed the formation of actomyosin cable at the A/P compartment boundary plays a key role in the establishment and maintenance of the boundary. But the molecules regulating the formation of the actomyosin cable are still unknown. Therefore, in this study we used RNAi to screen a wide-range of actin-associated actin-binding proteins and observed whether A/P boundary is affected. Although lose of function of some actin-binding proteins caused deformation of wing disc, none of these can interfere the smoothness of the A/P boundary. The result implies the effect of a single actin-binding protein might not efficient to influent boundary formation.