Abstract
增生細胞核抗原（ＰＣＮＡ）為ＤＮＡ聚合酵素d的輔助蛋白，參與真核細胞細胞內ＤＮＡ複製與切除修補。先前研究報告指出大白鼠增生細胞核抗原啟動子具有血清反應性與紫外線誘發性現象，本論文利用重疊聚合鏈反應方法在大白鼠增生細胞核抗原啟動子建構特定AP-1位置的突變啟動子，研究大白鼠增生細胞核抗原啟動子中的AP-1位置(在-64到-58)對血清反應性與紫外線誘發性的影響，AP-1突變啟動子與野生啟動子比較，活性明顯降低。而且，當把AP-1突變啟動子穩定轉殖進入細胞後，從實驗數據發現AP-1突變啟動子喪失部分血清反應性與紫外線誘發性。實驗結果顯示大白鼠增生細胞核抗原啟動子中特定AP-1位置(從-64到-58)是基因轉錄所必須，並在血清刺激與紫外線照射反應上扮演重要角色。Proliferating cell nuclear antigen (PCNA), anauxiliary factorof DNA polymerase D, is required in DNA replication and excisionrepair. Previous studies have shown that the rat PCNA promoterpossesses the serum responsiveness and UV inducibility.In thisthesis, the role of AP-1 site (at -64/-58) in rat PCNA promoterin the aspects of serum responsiveness and UV inducibility wasstudied. The site-specific AP-1 mutant of rat PCNA promoter wasconstructed using overlap PCR method. The AP-1 mutant promoterisremarkably less active in comparison with itsrespective wildtype promoter. Furthermore, the AP-1 mutant partially loses theserum responsiveness and UVinducibility in the experiments inwhich the mutant promoter was stably transfected into cells.Resultsof this thesis study suggest that AP-1 site (at -64/-58)is necessary for basal activity and plays significant role serumstimulation and UV induction of the rat PCNA promoter.