Abstract
Recently, researches about manipulation and analysis of bio-particles by MEMS technology are growing because of the fabrication development, and it is an important field ob bio-technology. The main purpose is to integrate the functions we need into a Lab-On-a-Chip system, and the chips have the function of bio-detection and analysis. Taking the micro-device of cell sorting for example, there are many technology have been presented, such as microgripper. But each of them has some restrications. Therefore, we want to design a bio-chip which could separate cells easily and efficiently. In this thesis, we use the characteristic of microfluid and design the bio-chip to combine with DEP (dielectrophoresis) and microchannel to sort cells. By collecting the two fluids with different flow rates, all cells will close the wall in the microchannel. At the same time, the cells will separate preliminary because of the sizes of the cells and the drag force. By applying input AC voltage within specific frequency range on the micro electrodes, cells could be separate and collect with negative DEP force. We through numerical simulation software, CFDRC, and the design concepts are realized by MEME fabrication process. At last, we use polystyrene beads in place of cells to demonstrate the functions of our chip.