Abstract
With the breakthrough in microscopy, we can obtain the neuron connection in the brain at either macroscale or microscale. The goal of neuroscientists is to understand how the propagation, storage, and acquisition of information are accomplished in the billions of nerve cells. First, we need to establish the wiring diagram of the brain, a three-dimensional brain mapping, the neuronal connection based on the structure and the function of nerve cells. In our lab, we will provide a synchrotron X-ray based whole-head mapping with Golgi stained specimen. The Golgi-Cox method provides a fast staining process but incomplete stained neurons as well. For the improvement of the staining quality, we establish a new protocol based on optimal experimental temperature, incubation time, fixation condition and additive. To speed the optimization of Golgi method, we decided to primarily image samples through optical microscopy with clearing agent, thioglycerol. Synchrotron X-ray based brain mapping with metallic stain technique has drawn the attention of neuroscientists. The present study introduced the potential orientations of Golgi method optimization and fast approach to screen out by clearing tissue.