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Elucidation of the Mechanisms of Glucose Starvation on Mitochondria Dynamics in Yeast Cells
Thesis

Elucidation of the Mechanisms of Glucose Starvation on Mitochondria Dynamics in Yeast Cells

Huang, Hsin Wei
Masters, 國立清華大學, 生物科技研究所
2014

Abstract

葡萄糖不足 粒線體動態平衡 白藜蘆醇 細胞衰老 glucose starvation mitochondrial dynamics senescence resveratrol
Mitochondria are highly dynamic organelles that constantly undergo fission/fusion processes in response to environment. The dynamics of mitochondria determines mitochondrial integrity and function. In mammalian cells, starvation leads to unopposed mitochondrial fusion and drives mitochondrial elongation. In yeast, glucose starvation also induces mitochondrial hyperfusion. However, the mechanism remains unclear. To clarify the relationship between glucose starvation and mitochondria morphology, I examined the mitochondrial dynamics by classifying morphology in WT, Δsnf1 and Δtor1 strains in nutrient-deprived medium. I found that SNF1 pathway involved in mitochondrial hyperfusion under glucose starvation condition. In addition, mitochondrial fusion and fission balance is also affected by senescence. Mitochondrial fragmentation was presented in yeast senescent cells and resveratrol can mitigate this phenomenon. My results demonstrated that that resveratrol reduces ratio cells with fragmented mitochondria is not through SNF1 or TOR1 pathway.

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