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Extracting Transcription Factor Binding Sites from Unaligned Gene Sequences with Statistical Models
Thesis

Extracting Transcription Factor Binding Sites from Unaligned Gene Sequences with Statistical Models

Wei-Hao Yuan
Masters, 國立清華大學, 電機工程學系
2005

Abstract

轉錄因子連結點 統計模型 貝氏網路 transcription factor binding site statistical model Bayesian networks
Transcription factor binding sites (motifs) are crucial in the regulation of the gene transcription. Recently, the chromatin immunoprecipitation followed by cDNA microarray hybridization (ChIP array) have been used to identify potential regulatory sequences, but the procedure can only map the probable protein-DNA interaction loci within 1-2 kilobases resolution. To find out the exact binding motifs, it is necessary to build a computational method to examine the ChIP-array binding sequences and search for possible motifs representing the transcription factor binding sites. In this thesis, we design a program to find out accurate motif sites in the yeast genome with dependency graphs and their expanded Bayesian networks. The program incorporates with the binomial probability model to build significant initial motif sets. Finally, we compare our results with those obtained from famous programs and show that our program outperforms these program in the consistence with known specificities.

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