Abstract
英文摘要 HIV-1 belongs to Retroviruses. HIV-1 expresses three basic components of retroviruses: Gag, Pol and Env. Gag protein can be devided into four major domains: MA, CA, NC amd p6. CA constitutes the core structure of a virus particle. In CA, previous research suggested that the region from MHR (major homology region) to the C terminus of CA is enough to produce a virus particle. Here, we showed that the sequence 1715-1758 is important for virus production. Of the ten CA mutants, Q179A, K182A and W184A caused severe defects in directing virus assembly and release. Although these mutants showed a subcellular distribution profile close to that of wild type, they appeared to be impaired to a certain degree in multimerization. Besides, we also found that different retroviruses may have similar structures from MHR to the region adjacent to C-terminus of MHR.