Abstract
To investigate host-pathogen interspecies interactions is a very important research topic in attempts to understand pathogenic and defensive mechanisms in the HIV infection process and help control the clinic pathogenic infections. In this study, with the help of simultaneously two-sided time-course HIV-human high throughput sequencing data (next generation sequencing NGS), RT-PCR data, epigenetic miRNA data and other omic data, the interspecies protein-protein miRNA interaction (PPMI) network is constructed for host and pathogen interactions in the HIV infection process. Principal interspecies PPMI networks are extracted as host-pathogen crosstalk network at different stages of HIV infection process. By comparing host-pathogen crosstalk networks in HIV infected cell with mock cell (without HIV infection) at different stages of HIV infection process respectively, host-pathogen crosstalk network markers at different infection stages are extracted. We further find the common core host-pathogen crosstalk network marker of the whole infection stage by the intersection of different stages host-pathogen crosstalk network markers. By further investigating this common core host-pathogen crosstalk network marker for molecular interaction shifting and viral proteins interaction in different HIV replication stages, we could get more insight into the pathologic mechanism of the HIV virus and the defense mechanism of human. This might offer the new way of drug target design for efficient therapies of AIDS. Keywords: HIV; miRNA; AIDS; interspecies protein-protein miRNA interaction (PPMI) network; host-pathogen crosstalk network mark; pathological and defense mechanism