Abstract
In order to understand the brain functions and apply to clinical neuroscience, the three-dimensional knowledge of the topography and localization of brain structures and how neuronal circuits to connection is very important. In insect, mushroom body is one of the best-studied compartments brain. Many studies indicated that mushroom body is the center for higher-order functions including olfactory learning, courtship behavior and visual learning. The broad understanding of the relationship between the map and behavior functions requires the adequate tools for high-resolution three-dimensional image. In this study, the fly brain was counterstained with a membrane probe-NBD ceramide and DID to l l neuropils, cleared by FocusClear and scanned by confocal microscopy to get a 3D high resolution image of fly brain. By the help of the model average software and warp technique of Amira® 3.0, a standard mushroom body was built and the images of the neuronal circuits from different samples were warped into the virtual standard mushroom body. As a result, a 3D image of standard mushroom body and a growing curve of the mushroom body in the developmental stage were presented and the neuronal circuits which associated with mushroom body from different samples were combined together. These high resolution circuit maps will help to understand how the neuronal circuits work and the role of mushroom body plays in the Drosophila.